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Annexin V-FITC/PI熒光雙染細胞凋亡檢測試劑盒
英文名：Annexin V-FITC/PI Apoptosis Kit
貨號：P-CA-201
價格：￥500 ￥950￥1500￥2400
- 20Assays
- 50Assays
- 100Assays
- 200Assays
產品概述
| 檢測原理 | Annexin V是一種鈣離子依賴性磷脂結合蛋白，與磷脂酰絲氨酸PS有高度親和力，可通過細胞外側暴露的PS與凋亡早期細胞胞膜結合，將Annexin V標記熒光染料，利用流式細胞儀檢測細胞凋亡。 細胞核染料（PI,DAPI,7-AAD）可與雙鏈DNA特異性結合，并產生強烈的熒光，正常情況下無法透過細胞膜。由于凋亡晚期或壞死細胞膜喪失完整性，細胞核染料（DAPI、PI、7-AAD）可進入細胞內對DNA進行染色，與Annexin V搭配使用，可區分處于不同凋亡時期的細胞。 |
| 應用類型 | 細胞凋亡檢測；Annexin V |
| 檢測方法 | 熒光 |
| 樣本類型 | 細胞 |
| 檢測時間 | 40 分鐘 |
| 檢測儀器 | 流式細胞儀 |
| 熒光 | FITC |
| 激發/發射波長(EX/Em(nm)) | 490/530 |
| 流式儀通道設置 | FITC |
| 自備試劑 | PBS(PB180327), 去離子水 |
| 運輸條件 | 冰袋 |
| 保藏溫度 | 2~8℃，避光保存 |
| 保質期 | 12個月 |
產品組分
參考文獻
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